ARG10684

anti-Hsp 27 antibody

anti-Hsp 27 antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Cow,Horse,Pig,Monkey

Overview

Product Description Chicken Polyclonal antibody recognizes Hsp 27
Tested Reactivity Hu, Cow, Hrs, Mk, Pig
Predict Reactivity Chk
Species Does Not React With Ms, Rat
Tested Application ICC/IF, IHC-Fr, WB
Host Chicken
Clonality Polyclonal
Isotype IgY
Target Name Hsp 27
Immunogen Recombinant full length purified HSP27 from E. coli.
Conjugation Un-conjugated
Full Name heat shock 27kDa protein 1
Alternate Names HSP 27; Heat shock 27 kDa protein; HMN2B; HS.76067; SRP27; HEL-S-102; HspB1; CMT2F; 28 kDa heat shock protein; HSP27; Heat shock protein beta-1; Hsp25; Estrogen-regulated 24 kDa protein; Stress-responsive protein 27; HSP28

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:2000
IHC-Fr1:2000
WB1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3315 Human HSPB1

Swiss-port # P04792 Human Heat shock protein beta-1

Gene Symbol HSPB1
Gene Full Name heat shock 27kDa protein 1
Background The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]
Function Involved in stress resistance and actin organization. [UniProt]
PTM Phosphorylated upon exposure to protein kinase C activators and heat shock (PubMed:8325890). Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement (PubMed:1332886, PubMed:8093612, PubMed:19166925).

Images (4) Click the Picture to Zoom In

  • ARG10684 anti-Hsp 27 antibody ICC/IF image

    Immunocytochemistry: HeLa cells stained with ARG10684 anti-Hsp 27 antibody (green), and co-stained with a monoclonal antibody to High mobility Group B protein 1 (HMGB1, red) and DNA (blue). ARG10684 reveals strong cytoplasmic staining and penetrates into the actin rich ruffled margins, while the HMGB1 antibody reveals strong nuclear staining which overlaps with the DNA staining.

  • ARG10684 anti-Hsp 27 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG10684 anti-Hsp 27 antibody (green) at 1:1000 dilution and costained with Mouse mAb to Hsp 60 (red) at 1:5000 dilution. DAPI (blue) for nuclear staining.

    The Hsp 27 antibody produces strong cytoplasmic staining, while the Hsp 60 antibody specifically labels mitochondria.

  • ARG10684 anti-Hsp 27 antibody WB image

    Western blot: HeLa cell crude extracts stained with ARG10684 anti-Hsp 27 antibody.

  • ARG10684 anti-Hsp 27 antibody WB image

    Western blot: NIH/3T3, C6, HeLa, HEK293, SH-SY5Y and COS-1 cell lysates stained with ARG10684 anti-Hsp 27 antibody (red) at 1:2000 dilution. The blot was simultaneously stained with Mouse mAb to beta tubulin (green) at 1:10000 dilution.

    Note that the antibody does not recognize rodent Hsp 27.