ARG22196
anti-Hsp 40 antibody
anti-Hsp 40 antibody for ICC/IF,Immunoprecipitation,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Hsp 40 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IP, WB |
| Specificity | Detects ~40kDa. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Target Name | Hsp 40 |
| Antigen Species | Human |
| Immunogen | Recombinant purified Human HSP40 |
| Conjugation | Un-conjugated |
| Alternate Names | Hsp40; HSP40; DnaJ protein homolog 1; hDj-1; HSPF1; Human DnaJ protein 1; Hdj1; RSPH16B; Heat shock 40 kDa protein 1; DnaJ homolog subfamily B member 1; Heat shock protein 40; Sis1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Rabbit antiserum. |
| Buffer | Rabbit Antiserum |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DNAJB1 |
| Gene Full Name | DnaJ (Hsp40) homolog, subfamily B, member 1 |
| Background | This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| Function | Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. [UniProt] |
| Cellular Localization | Cytoplasm, Nucleus |
| Calculated MW | 38 kDa |
Images (3) Click the Picture to Zoom In
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ARG22196 anti-Hsp 40 antibody ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22196 anti-Hsp 40 antibody (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
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ARG22196 anti-Hsp 40 antibody WB image
Western blot: Human HeLa cell lysates stained with ARG22196 anti-Hsp 40 antibody at 1:1000 dilution.
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ARG22196 anti-Hsp 40 antibody ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22196 anti-Hsp 40 antibody (red) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
