ARG10757

anti-Hsp 60 antibody

anti-Hsp 60 antibody for ICC/IF,IHC-Frozen sections,Western blot and Human

Controls and Markers antibody; Signaling Transduction antibody; Mitochondrial Marker antibody

Overview

Product Description Chicken Polyclonal antibody recognizes Hsp 60
Tested Reactivity Hu
Tested Application ICC/IF, IHC-Fr, WB
Host Chicken
Clonality Polyclonal
Isotype IgY
Target Name Hsp 60
Species Human
Immunogen Recombinant full length purified HSP60 from E. coli.
Conjugation Un-conjugated
Full Name heat shock 60kDa protein 1 (chaperonin)
Alternate Names Heat shock protein 60; HuCHA60; 60 kDa heat shock protein, mitochondrial; GROEL; P60 lymphocyte protein; 60 kDa chaperonin; HSP-60; Mitochondrial matrix protein P1; HLD4; SPG13; HSP60; Chaperonin 60; Hsp60; CPN60; HSP65

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:2000 - 1:5000
IHC-Fr1:2000 - 1:5000
WB1:10000 - 1:20000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 61 kDa

Properties

Form Liquid
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3329 Human HSPD1

Swiss-port # P10809 Human 60 kDa heat shock protein, mitochondrial

Gene Symbol HSPD1
Gene Full Name heat shock 60kDa protein 1 (chaperonin)
Background This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
Function Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. [UniProt]
Highlight Related products:
Hsp60 antibodies; Hsp60 Duos / Panels; Anti-Chicken IgY secondary antibodies;
Related poster download:
Organelle Markers & Loading Control
Research Area Controls and Markers antibody; Signaling Transduction antibody; Mitochondrial Marker antibody

Images (3) Click the Picture to Zoom In

  • ARG10757 anti-Hsp 60 antibody ICC/IF image

    Immunocytochemistry: HeLa cells were stained with ARG10757 anti-Hsp 60 antibody (green). Blue is DAPI staining of DNA. ARG10757 reveals strong staining in mitochondria.

  • ARG10757 anti-Hsp 60 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG10757 anti-Hsp 60 antibody (green) at 1:5000 dilution and costained with Mouse mAb to actin (red) at 1:500 dilution. DAPI (blue) for nuclear staining.

    The Hsp 60 antibody produces strong and specific staining of mitochondria, while the actin antibody labels the submembranous cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.

  • ARG10757 anti-Hsp 60 antibody WB image

    Western blot: 1) SHSY-5Y, and 2) HeLa cell lysates stained with ARG10757 anti-Hsp 60 antibody at 1: 20,000 dilution.