ARG22208

anti-Hsp 70 / Hsc 70 antibody [N27F3-4]

anti-Hsp 70 / Hsc 70 antibody [N27F3-4] for Electron microscopy,Flow cytometry,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Bovine,C. elegans,Chicken,D. melanogaster ,Dog,Fish,Guinea pig,Hamster,Human,Monkey,Mouse,Pig,Plant,Rabbit,Rat,Sheep,Xenopus laevis

Overview

Product Description Mouse Monoclonal antibody [N27F3-4] recognizes Hsp 70 / Hsc 70
Tested Reactivity Hu, Ms, Rat, Bov, Ce, Chk, Dm, Dog, Fsh, Gpig, Hm, Mk, Pig, Plnt, Rb, Sheep, Xenopus laevis
Tested Application EM, FACS, ICC/IF, IHC, IP, WB
Specificity Detects a band of ~70-73kDa. It Detects Hsp70 and Hsc70.
Host Mouse
Clonality Monoclonal
Clone N27F3-4
Isotype IgG
Target Name Hsp 70 / Hsc 70
Antigen Species Human
Immunogen Recombinant Hsp70
Conjugation Un-conjugated
Alternate Names Heat shock 70 kDa protein 1A; HSPA1; HSP70I; Heat shock 70 kDa protein 1; HSP70-1A; HEL-S-103; HSP70.1; HSP72; HSP70-1

Application Instructions

Application Suggestion
Tested Application Dilution
EMAssay-dependent
FACSAssay-dependent
ICC/IF1:50
IHC1:100
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.2), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 193740 Mouse HSPA1A

GeneID: 281825 Bovine HSPA1A

GeneID: 282254 Bovine HSPA1A

Gene Symbol HSPA1A
Gene Full Name heat shock 70kDa protein 1A
Background This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]
Function In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation. [UniProt]
Cellular Localization Cytoplasm
Calculated MW ~72 (Hsp) and ~73 kDa (Hsc).
PTM In response to cellular stress, acetylated at Lys-77 by NA110 and then gradually deacetylated by HDAC4 at later stages. Acetylation enhances its chaperone activity and also determines whether it will function as a chaperone for protein refolding or degradation by controlling its binding to co-chaperones HOPX and STUB1. The acetylated form and the non-acetylated form bind to HOPX and STUB1 respectively. Acetylation also protects cells against various types of cellular stress.

Images (1) Click the Picture to Zoom In

  • ARG22208 anti-Hsp 70 / Hsc 70 antibody [N27F3-4] WB image

    Western blot: 15 µg of Human cell lysates stained with ARG22208 anti-Hsp 70 / Hsc 70 antibody [N27F3-4] at 1:1000 dilution (2 hours at RT).