anti-Hsp 70 antibody (N-term)

anti-Hsp 70 antibody (N-term) for ICC/IF,Western blot and Human

Cancer antibody; Signaling Transduction antibody


Product Description Mouse Monoclonal antibody recognizes Hsp 70
Tested Reactivity Hu
Tested Application ICC/IF, WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name Hsp 70
Antigen Species Human
Immunogen Purified recombinant human Hsp70 (N-terminus) protein fragments expressed in E. coli.
Conjugation Un-conjugated
Alternate Names Heat shock 70 kDa protein 1A; HSPA1; HSP70I; Heat shock 70 kDa protein 1; HSP70-1A; HEL-S-103; HSP70.1; HSP72; HSP70-1

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 70 kDa


Form Liquid
Purification Affinity purified
Buffer 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol
Preservative 0.2% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 3303 Human HSPA1A

Swiss-port # P0DMV8 Human Heat shock 70 kDa protein 1A

Gene Symbol HSPA1A
Gene Full Name heat shock 70kDa protein 1A
Background This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]
Function In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation. [UniProt]
Cellular Localization Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. [UniProt]
Research Area Cancer antibody; Signaling Transduction antibody
Calculated MW 70 kDa
PTM In response to cellular stress, acetylated at Lys-77 by NA110 and then gradually deacetylated by HDAC4 at later stages. Acetylation enhances its chaperone activity and also determines whether it will function as a chaperone for protein refolding or degradation by controlling its binding to co-chaperones HOPX and STUB1. The acetylated form and the non-acetylated form bind to HOPX and STUB1 respectively. Acetylation also protects cells against various types of cellular stress. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG54020 anti-Hsp 70 antibody (N-term) ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG54020 anti-Hsp 70 antibody (N-term) at 1:300 dilution.

  • ARG54020 anti-Hsp 70 antibody (N-term) WB image

    Western blot: HeLa cell lysate stained with ARG54020 anti-Hsp 70 antibody (N-term) at 1:1000 dilution.