anti-Hsp 90 beta phospho (Ser254) antibody

anti-Hsp 90 beta phospho (Ser254) antibody for Western blot and Human,Mouse,Rat

Cancer antibody; Signaling Transduction antibody


Product Description Rabbit Polyclonal antibody recognizes Hsp 90 beta phospho (Ser254)
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Hsp 90 beta
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of serine 254 (V-G-S(p)-D-E) derived from Human HSP90B.
Conjugation Un-conjugated
Alternate Names HSPC2; D6S182; Heat shock 84 kDa; HSP90B; HSP84; HSP 84; Heat shock protein HSP 90-beta; HSP 90; HSPCB

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 15516 Mouse HSP90AB1

GeneID: 301252 Rat HSP90AB1

GeneID: 3326 Human HSP90AB1

Gene Symbol HSP90AB1
Gene Full Name heat shock protein 90kDa alpha (cytosolic), class B member 1
Background Molecular chaperone. Has ATPase activity.
Function Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. [UniProt]
Research Area Cancer antibody; Signaling Transduction antibody
Calculated MW 83 kDa
PTM Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
S-nitrosylated; negatively regulates the ATPase activity.
Phosphorylation at Tyr-301 by SRC is induced by lipopolysaccharide (PubMed:23585225). Phosphorylation at Ser-226 and Ser-255 inhibits AHR interaction (PubMed:15581363).
Methylated by SMYD2; facilitates dimerization and chaperone complex formation; promotes cancer cell proliferation.
Cleaved following oxidative stress resulting in HSP90AB1 protein radicals formation; disrupts the chaperoning function and the degradation of its client proteins.

Images (2) Click the Picture to Zoom In

  • ARG51690 anti-Hsp 90 beta phospho (Ser254) antibody WB image

    Western blot: Extracts from HeLa cells untreated(lane 1) or treated with hot shock(lane 2) stained with ARG51690 anti-Hsp 90 beta phospho (Ser254) antibody.

  • ARG51690 anti-Hsp 90 beta phospho (Ser254) antibody WB image

    Western blot: Extracts from HeLa cells, treated with calf intestinal phosphatase (CIP), stained with ARG51690 anti-Hsp 90 beta phospho (Ser254) antibody.