ARG54856

anti-IFITM3 / Fragilis antibody

anti-IFITM3 / Fragilis antibody for ICC/IF,Western blot and Human

Developmental Biology antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes IFITM3 / Fragilis
Tested Reactivity Hu
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IFITM3 / Fragilis
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 1-30 (N-terminus) of Human Interferon-inducible protein (IFITM3).
Conjugation Un-conjugated
Alternate Names IP15; DSPA2b; 1-8U; Interferon-induced transmembrane protein 3; Dispanin subfamily A member 2b; Interferon-inducible protein 1-8U

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:500
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HT1080

Properties

Form Liquid
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10410 Human IFITM3

Swiss-port # Q01628 Human Interferon-induced transmembrane protein 3

Gene Symbol IFITM3
Gene Full Name interferon induced transmembrane protein 3
Background The protein encoded by this gene is an interferon-induced membrane protein that helps confer immunity to influenza A H1N1 virus, West Nile virus, and dengue virus. Two transcript variants, only one of them protein-coding, have been found for this gene. Another variant encoding an N-terminally truncated isoform has been reported, but the full-length nature of this variant has not been determined. [provided by RefSeq, May 2012]
Function IFN-induced antiviral protein which disrupts intracellular cholesterol homeostasis. Inhibits the entry of viruses to the host cell cytoplasm by preventing viral fusion with cholesterol depleted endosomes. May inactivate new enveloped viruses which buds out of the infected cell, by letting them go out with a cholesterol depleted membrane. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV) and Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and vesicular stomatitis virus (VSV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV S protein-mediated viral entry and VSV G protein-mediated viral entry. Plays a critical role in the structural stability and function of vacuolar ATPase (v-ATPase). Establishes physical contact with the v-ATPase of endosomes which is critical for proper clathrin localization and is also required for the function of the v-ATPase to lower the pH in phagocytic endosomes thus establishing an antiviral state. [UniProt]
Cellular Localization Cell membrane; Single-pass type II membrane protein. Late endosome membrane; Single-pass type II membrane protein. Lysosome membrane; Single-pass type II membrane protein
Research Area Developmental Biology antibody
Calculated MW 15 kDa
PTM Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus.
Not glycosylated.
Polyubiquitinated with both 'Lys-48' and 'Lys-63' linkages. Ubiquitination negatively regulates antiviral activity. Lys-24 is the most prevalent ubiquitination site.

Images (2) Click the Picture to Zoom In

  • ARG54856 anti-IFITM3 / Fragilis antibody ICC/IF image

    Immunofluorescence: HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then stained with ARG54856 anti-IFITM3 / Fragilis antibody (green) at 1:200 dilution, 2 h at room temperature. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 µM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min). Note the highly specific localization of the IFITM3 immunoreactivity to the Golgi.

  • ARG54856 anti-IFITM3 / Fragilis antibody WB image

    Western blot: 35 µg of HT1080 cell lysate stained with ARG54856 anti-IFITM3 / Fragilis antibody at 1:1000 dilution.