ARG21504

anti-IFN gamma antibody [XMG1.2] (Biotin)

anti-IFN gamma antibody [XMG1.2] (Biotin) for ELISA,ELISPOT,Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Western blot,Neutralizing and Mouse

Overview

Product Description Biotin-conjugated Rat Monoclonal antibody [XMG1.2] recognizes IFN gamma
Tested Reactivity Ms
Tested Application ELISA, ELISPOT, FACS, ICC/IF, IHC-Fr, IHC-P, Neut, WB
Specificity Mouse IFN-γ.
Host Rat
Clonality Monoclonal
Clone XMG1.2
Isotype IgG1, kappa
Target Name IFN gamma
Antigen Species Mouse
Immunogen E. coli-expressed IFN-γ
Conjugation Biotin
Full Name interferon gamma
Alternate Names IFN-gamma; Interferon gamma; Immune interferon; IFG; IFI

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:2000 - 1:4000
ELISPOTAssay-dependent
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
NeutAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 19 kDa

Properties

Form Liquid
Buffer PBS and Sodium azide
Preservative Sodium azide
Concentration 0.5 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IFNG
Gene Full Name interferon gamma
Background This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]
Function Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt]
PTM Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.