ARG63880

anti-IFT88 / Polaris antibody

anti-IFT88 / Polaris antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes IFT88 / Polaris
Tested Reactivity Hu, Ms
Predict Reactivity Dog, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Specificity This antibody is expected to recognise both reported isoforms (NP_783195.2; NP_006522.2)
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IFT88 / Polaris
Species Human
Immunogen C-KKRIDEDDFADEE
Conjugation Un-conjugated
Alternate Names Recessive polycystic kidney disease protein Tg737 homolog; hTg737; D13S1056E; Intraflagellar transport protein 88 homolog; TG737; TPR repeat protein 10; TTC10; Tetratricopeptide repeat protein 10; DAF19

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µg/ml
ICC/IF10 µg/ml
IHC-P3 µg/ml
WB0.3 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 94 kDa

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 21821 Mouse IFT88

GeneID: 8100 Human IFT88

Swiss-port # Q13099 Human Intraflagellar transport protein 88 homolog

Swiss-port # Q61371 Mouse Intraflagellar transport protein 88 homolog

Background This gene encodes a member of the tetratrico peptide repeat (TPR) family. Mutations of a similar gene in mouse can cause polycystic kidney disease. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Highlight Related products:
Anti-Goat IgG secondary antibodies;
Related news:
Gene therapy for retinitis pigmentosa (RP)
Research Area Gene Regulation antibody

Images (5) Click the Picture to Zoom In

  • ARG63880 anti-IFT88 / Polaris antibody ICC/IF image

    Immunofluorescence: Paraformaldehyde fixed HepG2 cells permeabilized with 0.15% Triton. Cells were stained with ARG63880 anti-IFT88 / Polaris antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.

  • ARG63880 anti-IFT88 / Polaris antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Kidney. (Microwaved antigen retrieval with Tris/EDTA buffer pH9) stained with ARG63880 anti-IFT88 / Polaris antibody at 3 µg/ml dilution followed by HRP-staining.

  • ARG63880 anti-IFT88 / Polaris antibody WB image

    Western blot: 35 µg of Human kidney lysate (in RIPA buffer) stained with ARG63880 anti-IFT88 / Polaris antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.

  • ARG63880 anti-IFT88 / Polaris antibody FACS image

    Flow Cytometry: Paraformaldehyde-fixed HepG2 cells permeabilized with 0.5% Triton. Cells were stained with ARG63880 anti-IFT88 / Polaris antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).

  • ARG63880 anti-IFT88 / Polaris antibody WB image

    Western blot: 35 µg of HepG2 (A) and NIH/3T3 (B) cell lysates (in RIPA buffer) stained with ARG63880 anti-IFT88 / Polaris antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.