ARG54710

anti-IKB alpha antibody

anti-IKB alpha antibody for Western blot and Human,Mouse,Rat

Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody; IκB alpha degradation Study antibody; NF-kB Activation Study antibody
publication_link Publication1

Overview

Product Description

Rabbit Polyclonal antibody recognizes IKB alpha

Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IKB alpha
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 1-30 of Human IKB alpha (NP_065390.1).
Conjugation Un-conjugated
Alternate Names NFKBI; I-kappa-B-alpha; IkB-alpha; NF-kappa-B inhibitor alpha; Major histocompatibility complex enhancer-binding protein MAD3; IKBA; IkappaBalpha; MAD-3

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Purification Protein G purified
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18035 Mouse NFKBIA

GeneID: 25493 Rat NFKBIA

GeneID: 4792 Human NFKBIA

Gene Symbol NFKBIA
Gene Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Background This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
Function Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. [From Uniprot]
Cellular Localization Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
Highlight Related Antibody Duos and Panels:
ARG30255 IKB alpha degradation Antibody Panel (IKK alpha, IKK beta, IKB alpha)
Related products:
IKB alpha antibodies; IKB alpha Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related poster download:
The NF-kappa B Pathways.pdf
Toll-like Receptor.pdf
Research Area Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody; IκB alpha degradation Study antibody; NF-kB Activation Study antibody
Calculated MW 36 kDa
PTM Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.

Images (1) Click the Picture to Zoom In

  • ARG54710 anti-IKB alpha antibody WB image

    Western blot: 30 µg of HeLa cells treated with 5, 10 or 20 ng/ml of TNF alpha at 10, 20 or 30 mins. The blots were stained with ARG54710  anti-IKB alpha antibody at 1:500 dilution. The ARG10112 anti-GAPDH antibody [6C5] was used as loading control.

Specific References

Caffeic acid phenethyl ester down-regulates claudin-2 expression at the transcriptional and post-translational levels and enhances chemosensitivity to doxorubicin in lung adenocarcinoma A549 cells.

WB / Human

Sonoki Hiroyuki et al.
The Journal of Nutritional Biochemistry.,  (2018)

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