ARG51704

anti-IKB epsilon phospho (Ser22) antibody

anti-IKB epsilon phospho (Ser22) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse

Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes IKB epsilon phospho (Ser22)
Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IKB epsilon
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of serine 22 (I-E-S(p)-L-R) derived from Human IkB-ε.
Conjugation Un-conjugated
Alternate Names IkB-epsilon; NF-kappa-BIE; IkappaBepsilon; NF-kappa-B inhibitor epsilon; IkB-E; IKBE; I-kappa-B-epsilon

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18037 Mouse NFKBIE

GeneID: 4794 Human NFKBIE

Swiss-port # O00221 Human NF-kappa-B inhibitor epsilon

Swiss-port # O54910 Mouse NF-kappa-B inhibitor epsilon

Gene Symbol NFKBIE
Gene Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon
Background Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
Function Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes. [UniProt]
Research Area Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 53 kDa
PTM Serine phosphorylated; followed by proteasome-dependent degradation.

Images (2) Click the Picture to Zoom In

  • ARG51704 anti-IKB epsilon phospho (Ser22) antibody WB image

    Western blot: Extracts from 293 cells untreated or treated with TNF stained with ARG51704 anti-IKB epsilon phospho (Ser22) antibody.

  • ARG51704 anti-IKB epsilon phospho (Ser22) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51704 anti-IKB epsilon phospho (Ser22) antibody (left) or the same antibody preincubated with blocking peptide (right).