ARG66320

anti-IKK gamma antibody [SQab1868]

anti-IKK gamma antibody [SQab1868] for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry,Immunoprecipitation and Human,Mouse,Rat

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab1868] recognizes IKK gamma
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, IP, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1868
Isotype IgG
Target Name IKK gamma
Antigen Species Human
Immunogen Synthetic peptide around the N-terminus of IKK gamma.
Conjugation Un-conjugated
Full Name inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
Alternate Names I-kappa-B kinase subunit gamma; IkB kinase-associated protein 1; Fip3p; NF-kappa-B essential modifier; IKK-gamma; IP; NF-kappa-B essential modulator; NEMO; ZC2HC9; AMCBX1; IP2; IKKG; IP1; FIP3; IMD33; IkB kinase subunit gamma; IKKAP1; IPD2; FIP-3; Inhibitor of nuclear factor kappa-B kinase subunit gamma

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:200 - 1:1000
IHC-P1:100 - 1:200
IP1:50
WB1:2000 - 1:5000
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 48 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 16151 Mouse IKBKG

GeneID: 309295 Rat IKBKG

GeneID: 8517 Human IKBKG

Gene Symbol IKBKG
Gene Full Name inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
Background IKK gamma is a regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Mar 2016]
Function IKK gamma: Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'-linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination. [UniProt]
PTM Phosphorylation at Ser-68 attenuates aminoterminal homodimerization.

Polyubiquitinated on Lys-285 through 'Lys-63'; the ubiquitination is mediated by NOD2 and RIPK2 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Polyubiquitinated on Lys-399 through 'Lys-63'; the ubiquitination is mediated by BCL10, MALT1 and TRAF6 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Monoubiquitinated on Lys-277 and Lys-309; promotes nuclear export. Polyubiquitinated through 'Lys-27' by TRIM23; involved in antiviral innate and inflammatory responses. Linear polyubiquitinated on Lys-111, Lys-143, Lys-226, Lys-246, Lys-264, Lys-277, Lys-285, Lys-292, Lys-302, Lys-309 and Lys-326; the head-to-tail polyubiquitination is mediated by the LUBAC complex and plays a key role in NF-kappa-B activation. Polyubiquitinated on Lys-309 and Lys-321 via 'Lys-27'-linked ubiquitin by Shigella flexneri E3 ubiquitin-protein ligase ipah9.8, leading to its degradation by the proteasome. Deubiquitinated by USP10 in a TANK-dependent and -independent manner, leading to the negative regulation of NF-kappaB signaling upon DNA damage (PubMed:25861989).

Sumoylated on Lys-277 and Lys-309 with SUMO1; the modification results in phosphorylation of Ser-85 by ATM leading to a replacement of the sumoylation by mono-ubiquitination on these residues.

Neddylated by TRIM40, resulting in stabilization of NFKBIA and down-regulation of NF-kappa-B activity. [UniProt]

Images (5) Click the Picture to Zoom In

  • ARG66320 anti-IKK gamma antibody [SQab1868] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66320 anti-IKK gamma antibody [SQab1868] (red) at 1:1,000 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

  • ARG66320 anti-IKK gamma antibody [SQab1868] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded liver tissue stained with ARG66320 anti-IKK gamma antibody [SQab1868] at 1:200 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.

  • ARG66320 anti-IKK gamma antibody [SQab1868] WB image

    Western blot: 10 µg of Jurkat, K562, HeLa and NIH/3T3 cell lysates stained with ARG66320 anti-IKK gamma antibody [SQab1868] at 1:2000 dilution.

  • ARG66320 anti-IKK gamma antibody [SQab1868] IP image

    Immunoprecipitation: 0.4 mg of HeLa whole cell lysate immunoprecipitated (1:50) and stained with ARG66320 anti-IKK gamma antibody [SQab1868]. 1) ARG66320 IP in HeLa whole cell, 2) PBS instead of ARG66320 in HeLa whole cell lysate, and 3) HeLa whole cell lysate, 10 µg (input).

  • ARG66320 anti-IKK gamma antibody [SQab1868] WB image

    Western blot: 10 µg of PC-12, PC-3, HepG2 and Mouse kidney lysates stained with ARG66320 anti-IKK gamma antibody [SQab1868] at 1:2000 dilution.