ARG21422

anti-IL10 antibody [JES3-12G8] (Biotin)

anti-IL10 antibody [JES3-12G8] (Biotin) for ELISA,ELISPOT,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Neutralizing,Western blot and Human,Virus

Overview

Product Description

Biotin-conjugated Rat Monoclonal antibody [JES3-12G8 ] recognizes IL10

Tested Reactivity Hu, Virus
Tested Application ELISA, ELISPOT, IHC-Fr, IHC-P, Neut, WB
Specificity The IL10 antibody clone JES3-12G8 reacts with human interleukin-10 (IL-10) and viral IL-10. Might also react to Cynomolgus IL-10.
Host Rat
Clonality Monoclonal
Clone JES3-12G8
Isotype IgG2a, kappa
Target Name IL10
Antigen Species Human
Immunogen COS-expressed human IL-10
Conjugation Biotin
Full Name interleukin 10
Alternate Names IL10A; GVHDS; TGIF; IL-10; Cytokine synthesis inhibitory factor; CSIF; Interleukin-10

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:2000 - 1:4000
ELISPOTAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
NeutAssay-dependent
WBAssay-dependent
Application Note ELISA: The clone JES3-12G8 may be paired with the purified clone JES3-9D7 in a sandwich ELISA. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 21 kDa

Properties

Form Liquid
Buffer PBS and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 0.5 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3586 Human IL10

Swiss-port # P22301 Human Interleukin-10

Gene Symbol IL10
Gene Full Name interleukin 10
Background The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]
Function Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG21422 anti-IL10 antibody [JES3-12G8] (Biotin) standard curve image

    ELISA: Human IL-10 detected by ARG21419 anti-IL10 antibody [JES3-9D7] as capture antibody ( ARG21420: Azide free version), and ARG21422 anti-IL10 antibody [JES3-12G8] (Biotin) as detection antibody, follow by incubation with streptavidin-HRP.