ARG10031

anti-IL16 antibody [323]

anti-IL16 antibody [323] for ELISA and Human

Cell Biology and Cellular Response antibody; Gene Regulation antibody

Overview

Product Description

Mouse Monoclonal antibody [323] recognizes IL16

Tested Reactivity Hu
Tested Application ELISA
Specificity mAb 323 exhibits no detectable cross-reaction with human serum albumin, bovine serum albumin and other human cytokines or growth factors tested, such as IL-1β, IL-8, IL-7 MCAF, TGF-β1, EGF, bFGF, GM-CSF, M-CSF, TNF-α.
Host Mouse
Clonality Monoclonal
Clone 323
Isotype IgG2a,k
Target Name IL16
Antigen Species Human
Immunogen Purified recombinant human IL-16
Conjugation Un-conjugated
Alternate Names prIL-16; Lymphocyte chemoattractant factor; PRIL16; IL-16; NIL16; LCF; Pro-interleukin-16

Application Instructions

Application Note ELISA: The antibody matches with capture antibody clone 398 (Cat. No.: ARG10202) in sandwich ELISA.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2)
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3603 Human IL16

Swiss-port # Q14005 Human Pro-interleukin-16

Gene Symbol IL16
Gene Full Name interleukin 16
Background The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
Function Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.
Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells. [UniProt]
Resrarch Area Cell Biology and Cellular Response antibody; Gene Regulation antibody
PTM Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16.