ARG56697

anti-IL17 / IL17A antibody

anti-IL17 / IL17A antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human

Overview

Product Description Goat Polyclonal antibody recognizes IL17 / IL17A
Tested Reactivity Hu
Tested Application ELISA, IHC-P, Neut, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IL17 / IL17A
Antigen Species Human
Immunogen E.coli derived Recombinant Human IL-17 (IL-17A).
(MIVKAGITIP RNPGCPNSED KNFPRTVMVN LNIHNRNTNT NPKRSSDYYN RSTSPWNLHR NEDPERYPSV IWEAKCRHLG CINADGNVDY HMNSVPIQQE ILVLRREPPH CPNSFRLEKI LVSVGCTCVT PIVHHVA)
Conjugation Un-conjugated
Alternate Names IL-17A; Interleukin-17A; IL-17; CTLA8; IL17; CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: use 0.5 - 2.0 µg/ml as a capture antibody
IHC-P0.125 µg/ml - 0.25 µg/ml
Neut0.43 - 0.65 µg/ml (To yield [ND50] of the biological activity of Human IL - 17A (50.00 ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note Sandwich ELISA: Sandwich ELISA: Recommended antibody pairs in sandwich immunoassay for procalcitonin (capture - detection): ARG56697 - ARG56806
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3605 Human IL17A

Swiss-port # Q16552 Human Interleukin-17A

Gene Symbol IL17A
Gene Full Name interleukin 17A
Background The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]
Function Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. [UniProt]
Highlight Related products:
IL17 antibodies; IL17 ELISA Kits; IL17 recombinant proteins; Anti-Goat IgG secondary antibodies;
Related news:
HMGB1, a biomarker and therapeutic target in COVID-19
Calculated MW 18 kDa
PTM Found both in glycosylated and nonglycosylated forms.

Images (4) Click the Picture to Zoom In

  • ARG56697 anti-IL17 / IL17A antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56697 anti-IL17 / IL17A antibody concentration is 0.125 µg/ml - 0.25 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56697 anti-IL17 / IL17A antibody WB image

    Western blot: 250 - 0.24 ng of Human IL-17A stained with ARG56697 anti-IL17 / IL17A antibody, under reducing conditions.

  • ARG56697 anti-IL17 / IL17A antibody WB image

    Western blot: 250 - 0.24 ng of Human IL-17A stained with ARG56697 anti-IL17 / IL17A antibody, under non-reducing conditions.

  • ARG56697 anti-IL17 / IL17A antibody standard curve image

    Sandwich ELISA: ARG56697 anti-IL17 / IL17A antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with anti-IL17 / IL17A antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.