ARG21467

anti-IL5 antibody [TRFK5]

anti-IL5 antibody [TRFK5] for ELISA,ELISPOT,Flow cytometry,IHC-Frozen sections,ICC/IF,Purification,Neutralizing and Mouse

Overview

Product Description Rat Monoclonal antibody [TRFK5 ] recognizes IL5
Tested Reactivity Ms
Tested Application ELISA, ELISPOT, FACS, ICC/IF, IHC-Fr, Neut, Puri
Specificity Mouse/Human/Rhesus/Cynomolgus/Baboon/Sooty Mangabey IL-5.
Host Rat
Clonality Monoclonal
Clone TRFK5
Isotype IgG1, kappa
Target Name IL5
Antigen Species Mouse
Immunogen Semi-purified T cell clone supernatant
Conjugation Un-conjugated
Full Name interleukin 5
Alternate Names Eosinophil differentiation factor; EDF; IL-5; TRF; T-cell replacing factor; B-cell differentiation factor I; Interleukin-5

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA< 2 µg/ml
ELISPOTAssay-dependent
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
NeutAssay-dependent
PuriAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 15 kDa

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 16191 Mouse IL5

Swiss-port # P04401 Mouse Interleukin-5

Gene Symbol IL5
Gene Full Name interleukin 5
Background This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]
Function Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG21467 anti-IL5 antibody [TRFK5] standard curve image

    ELISA: Mouse IL-5 detected by ARG21467 anti-IL5 antibody [TRFK5] as capture antibody ( ARG21468: Azide free version), and ARG21470 anti-IL5 antibody [TRFK4] (Biotin) as detection antibody, follow by incubation with streptavidin-HRP.