ARG21474

anti-IL6 antibody [MP5-32C11] (Biotin)

anti-IL6 antibody [MP5-32C11] (Biotin) for ELISA,ELISPOT,ICC/IF,IHC-Frozen sections and Mouse

Overview

Product Description

Biotin-conjugated Rat Monoclonal antibody [MP5-32C11 ] recognizes IL6

Tested Reactivity Ms
Tested Application ELISA, ELISPOT, ICC/IF, IHC-Fr
Specificity Mouse IL-6.
Host Rat
Clonality Monoclonal
Clone MP5-32C11
Isotype IgG2a, kappa
Target Name IL6
Antigen Species Mouse
Immunogen COS-expressed mouse IL-6
Conjugation Biotin
Full Name interleukin 6
Alternate Names B-cell stimulatory factor 2; CDF; HSF; BSF-2; Interferon beta-2; IL-6; IFNB2; CTL differentiation factor; Interleukin-6; HGF; Hybridoma growth factor; BSF2; IFN-beta-2

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:1000 - 1:2000
ELISPOTAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 24 kDa

Properties

Form Liquid
Buffer PBS and Sodium azide
Preservative Sodium azide
Concentration 0.5 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 16193 Mouse IL6

Swiss-port # P08505 Mouse Interleukin-6

Gene Symbol IL6
Gene Full Name interleukin 6
Background This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]
Function Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. [UniProt]
PTM N- and O-glycosylated.

Images (1) Click the Picture to Zoom In

  • ARG21474 anti-IL6 antibody [MP5-32C11] (Biotin) standard curve image

    ELISA: Mouse IL-6 detected by ARG21471 anti-IL6 antibody [MP5-20F3] as capture antibody ( ARG21472: Azide free version), and ARG21474 anti-IL6 antibody [MP5-32C11] (Biotin) as detection antibody, follow by incubation with streptavidin-HRP.