ARG45758
anti-INSIG1 antibody
anti-INSIG1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes INSIG1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | INSIG1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human INSIG1. |
| Conjugation | Un-conjugated |
| Alternate Names | INSIG1; Insulin Induced Gene 1; Insulin-Induced Gene 1 Protein; INSIG-1 Membrane Protein; MGC1405; CL-6; INSIG-1; CL6 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 66 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | INSIG1 |
| Gene Full Name | Insulin Induced Gene 1 |
| Background | This gene encodes an endoplasmic reticulum membrane protein that regulates cholesterol metabolism, lipogenesis, and glucose homeostasis. The encoded protein has six transmembrane helices which contain an effector protein binding site. It binds the sterol-sensing domains of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase), and is essential for the sterol-mediated trafficking of these two proteins. It promotes the endoplasmic reticulum retention of SCAP and the ubiquitin-mediated degradation of HMG-CoA reductase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016] |
| Function | Oxysterol-binding protein that mediates feedback control of cholesterol synthesis by controlling both endoplasmic reticulum to Golgi transport of SCAP and degradation of HMGCR. [UniProt] |
| Cellular Localization | Endoplasmic reticulum; Membrane. [UniProt] |
| Calculated MW | 30 kDa |
| PTM | Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG45758 anti-INSIG1 antibody IHC-P image
Immunohistochemistry: Human liver cancer stained with ARG45758 anti-INSIG1 antibody at 2 μg/ml dilution.
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ARG45758 anti-INSIG1 antibody ICC/IF image
Immunofluorescence: Hela stained with ARG45758 anti-INSIG1 antibody at 5 μg/ml dilution.
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ARG45758 anti-INSIG1 antibody WB image
Western blot: HepG2 stained with ARG45758 anti-INSIG1 antibody at 0.5 μg/ml dilution.
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ARG45758 anti-INSIG1 antibody FACS image
Flow Cytometry: MCF-7 stained with ARG45758 anti-INSIG1 antibody at 1 µg/10^6 cells dilution.
