ARG10608

anti-IP3 Receptor antibody

anti-IP3 Receptor antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes IP3 Receptor
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IP3 Receptor
Antigen Species Human
Immunogen Recombinant fragment around aa. 2411-2758 of Human IP3 Receptor.
Conjugation Un-conjugated
Full Name inositol 1,4,5-trisphosphate receptor, type 1
Alternate Names IP3R; SCA29; InsP3R1; SCA15; Type 1 InsP3 receptor; SCA16; INSP3R1; PPP1R94; IP3R 1; IP3 receptor isoform 1; ACV; IP3R1; CLA4; Inositol 1,4,5-trisphosphate receptor type 1; Type 1 inositol 1,4,5-trisphosphate receptor

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P0.5 - 1 µg/ml
WB0.5 - 1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW ~ 314 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.025% Sodium azide and 2.5% BSA.
Preservative 0.025% Sodium azide
Stabilizer 2.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 16438 Mouse ITPR1

GeneID: 25262 Rat ITPR1

GeneID: 3708 Human ITPR1

Gene Symbol ITPR1
Gene Full Name inositol 1,4,5-trisphosphate receptor, type 1
Background This gene encodes an intracellular receptor for inositol 1,4,5-trisphosphate. Upon stimulation by inositol 1,4,5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in this gene cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Nov 2009]
Function Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1 (By similarity). Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways (By similarity). [UniProt]
PTM Phosphorylated on tyrosine residues.
Ubiquitination at multiple lysines targets ITPR1 for proteasomal degradation. Approximately 40% of the ITPR1-associated ubiquitin is monoubiquitin, and polyubiquitins are both 'Lys-48'- and 'Lys-63'-linked (By similarity).
Phosphorylated by cAMP kinase (PKA). Phosphorylation prevents the ligand-induced opening of the calcium channels. Phosphorylation by PKA increases the interaction with inositol 1,4,5-trisphosphate and decreases the interaction with AHCYL1.

Images (5) Click the Picture to Zoom In

  • ARG10608 anti-IP3 Receptor antibody IHC-P image

    Immunohistochemistry: Human lung cancer tissue stained with ARG10608 anti-IP3 Receptor antibody.

  • ARG10608 anti-IP3 Receptor antibody WB image

    Western blot: 1) Rat brain, 2) Rat liver, 3) HeLa, and 4) HepG2 lysates stained with ARG10608 anti-IP3 Receptor antibody.

  • ARG10608 anti-IP3 Receptor antibody IHC-P image

    Immunohistochemistry: Mouse brain tissue stained with ARG10608 anti-IP3 Receptor antibody.

  • ARG10608 anti-IP3 Receptor antibody IHC-P image

    Immunohistochemistry: Rat brain tissue stained with ARG10608 anti-IP3 Receptor antibody.

  • ARG10608 anti-IP3 Receptor antibody WB image

    Western blot: 0.5 ng of recombinant human protein stained with ARG10608 anti-IP3 Receptor antibody.