ARG54631

anti-IRAK4 antibody

anti-IRAK4 antibody for Western blot,ICC/IF and Human

Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody
nuts_pic
1

Overview

Product Description Rabbit Polyclonal antibody recognizes IRAK4
Tested Reactivity Hu
Tested Application ICC/IF, WB
Specificity This antibody specifically recognizeshuman IRAK-4 (IL-1 ReceptorAssociated Kinase-4). This antibodydoes not cross-react with other IRAKs.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IRAK4
Antigen Species Human
Immunogen A synthetic peptidecorresponding to amino acids at thecarboxy terminus of human IRAK-4.
Conjugation Un-conjugated
Alternate Names REN64; Renal carcinoma antigen NY-REN-64; NY-REN-64; Interleukin-1 receptor-associated kinase 4; EC 2.7.11.1; IRAK-4; IPD1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa and K562

Properties

Form Liquid
Purification purified by Immunoaffinity chromatography.
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51135 Human IRAK4

Swiss-port # Q9NWZ3 Human Interleukin-1 receptor-associated kinase 4

Gene Symbol IRAK4
Gene Full Name interleukin-1 receptor-associated kinase 4
Background IRAK-4 activates the NF-B and MAPKpathways and plays a role in IL-1Rmediated inflammatory responses andinnate immunity. IRAK-4-deficientanimals are resistant to challenge withLPS and are impaired in their responsesto viral and bacterial challenges.
Function Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections. [UniProt]
Highlight Related products:
IRAK4 antibodies; Anti-Rabbit IgG secondary antibodies;
Related poster download:
The NF-kappa B Pathways.pdf
Toll-like Receptor.pdf
Research Area Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 52 kDa
PTM Phosphorylated.

Images (3) Click the Picture to Zoom In

  • ARG54631 anti-IRAK4 antibody WB image

    Western Blot: IRAK-4 in HeLa cell lysate stained with ARG54631 anti-IRAK4 antibody at 1, 2, and 3μg/ml dilution.

  • ARG54631 anti-IRAK4 antibody ICC/IF image

    Immunofluorescence: K562 stained with ARG54631 anti-IRAK4 antibody at 10 μg/ml dilution.

  • ARG54631 anti-IRAK4 antibody ICC/IF image

    Immunofluorescence: K562 stained with ARG54631 anti-IRAK4 antibody at 10 μg/ml dilution.

Customer's Feedback

nuts_pic      Average

Review for anti-IRAK4 antibody

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC