anti-IRF4 / MUM1 antibody [SQab20188]

anti-IRF4 / MUM1 antibody [SQab20188] for IHC-Formalin-fixed paraffin-embedded sections and Human


Product Description Recombinant Rabbit Monoclonal antibody [SQab20188] recognizes IRF4 / MUM1
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SQab20188
Isotype IgG
Target Name IRF4 / MUM1
Antigen Species Human
Immunogen Synthetic peptide around the C-terminus of Human IRF4 / MUM1.
Conjugation Un-conjugated
Alternate Names LSIRF; MUM1; Multiple myeloma oncogene 1; SHEP8; Interferon regulatory factor 4; NF-EM5; Lymphocyte-specific interferon regulatory factor; IRF-4

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Diffuse large B cell lymphoma tissue.


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 3662 Human IRF4

Swiss-port # Q15306 Human Interferon regulatory factor 4

Gene Symbol IRF4
Gene Full Name interferon regulatory factor 4
Background The protein encoded by this gene belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. This family member is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2010]
Function Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity). [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 52 kDa
PTM Phosphorylation by ROCK2 regulates IL-17 and IL-21 production. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG66755 anti-IRF4 / MUM1 antibody [SQab20188] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human diffuse large B cell lymphoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66755 anti-IRF4 / MUM1 antibody [SQab20188] at 18°C - 25°C for 30 minutes.