ARG10643

anti-Insulin + Proinsulin antibody [D6C4]

anti-Insulin + Proinsulin antibody [D6C4] for ELISA,IHC-Frozen sections and Bovine,Human,Mouse,Pig,Rat

Overview

Product Description Mouse Monoclonal antibody [D6C4] recognizes Insulin + Proinsulin
Tested Reactivity Hu, Ms, Rat, Bov, Pig
Tested Application ELISA, IHC-Fr
Specificity This antibody cross-react with Human, Bovine and Pig insulin and proinsulin.
Host Mouse
Clonality Monoclonal
Clone D6C4
Isotype IgG1, kappa
Target Name Insulin + Proinsulin
Antigen Species Mouse
Immunogen Mouse insulin.
Conjugation Un-conjugated
Full Name insulin
Alternate Names IDDM; IDDM2; IDDM1; ILPR; MODY10; Insulin; IRDN

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
IHC-FrAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS (pH 7.4) and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3630 Human INS

GeneID: 397415 Pig INS

Swiss-port # P01308 Human Insulin

Swiss-port # P01315 Pig Insulin

Gene Symbol INS
Gene Full Name insulin
Background After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
Function Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. [UniProt]