anti-Intra Acrosomal Protein antibody [Hs-14] (FITC)

anti-Intra Acrosomal Protein antibody [Hs-14] (FITC) for Flow cytometry and Human,Mouse

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody


Product Description FITC-conjugated Mouse Monoclonal antibody [Hs-14] recognizes Intra Acrosomal Protein
Tested Reactivity Hu, Ms
Tested Application FACS
Specificity The clone Hs-14 reacts with a 220 kDa testis-specific human intra-acrosomal protein associated with the membranes of the acrosomal vesicle._x000D_
Host Mouse
Clonality Monoclonal
Clone Hs-14
Isotype IgM
Target Name Intra Acrosomal Protein
Antigen Species Human
Immunogen Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Conjugation FITC
Alternate Names D11S4365; Acrosomal vesicle protein 1; SP-10; Acrosomal protein SP-10; SPACA2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 100 µl of whole blood or 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
Buffer TBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 11451 Mouse ACRV1

GeneID: 56 Human ACRV1

Swiss-port # P26436 Human Acrosomal protein SP-10

Swiss-port # P50289 Mouse Acrosomal protein SP-10

Gene Symbol ACRV1
Gene Full Name acrosomal vesicle protein 1
Background One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Research Area Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody
Calculated MW 28 kDa

Images (1) Click the Picture to Zoom In

  • ARG53952 anti-Intra Acrosomal Protein antibody [Hs-14] (FITC) FACS image

    Flow Cytometry: Separation of unstained (black) and stained (red) human sperm cells. Cells were stained with ARG53952 anti-Intra Acrosomal Protein antibody [Hs-14] (FITC) at 3 µg/ml dilution.

Clone References

Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis.

Peknicova J et al.
Am J Reprod Immunol.,  (2005)




[Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization].

Chládek D et al.
Ceska Gynekol.,  (2000)