ARG51648

anti-JAK1 phospho (Tyr1022) antibody

anti-JAK1 phospho (Tyr1022) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes JAK1 phospho (Tyr1022)
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name JAK1
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of tyrosine 1022 (K-E-Y(p)-Y-T) derived from Human JAK1.
Conjugation Un-conjugated
Alternate Names JTK3; Janus kinase 1; JAK-1; Tyrosine-protein kinase JAK1; JAK1A; JAK1B; EC 2.7.10.2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3716 Human JAK1

Swiss-port # P23458 Human Tyrosine-protein kinase JAK1

Gene Symbol JAK1
Gene Full Name Janus kinase 1
Background Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
Function Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Signaling Transduction antibody
Calculated MW 133 kDa
PTM Autophosphorylated (PubMed:7615558). Phosphorylated on tyrosine residues in response to interferon gamma signaling (PubMed:7615558). Dephosphorylation of Tyr-1034 and Tyr-1035 by PTPN2 negatively regulates cytokine-mediated signaling (PubMed:11909529).
Ubiquitinated by RNF125; leading to its degradation by the proteasome.

Images (2) Click the Picture to Zoom In

  • ARG51648 anti-JAK1 phospho (Tyr1022) antibody WB image

    Western blot: Extracts from HT29 cells untreated or treated with UV stained with ARG51648 anti-JAK1 phospho (Tyr1022) antibody.

  • ARG51648 anti-JAK1 phospho (Tyr1022) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51648 anti-JAK1 phospho (Tyr1022) antibody (left) or the same antibody preincubated with blocking peptide (right).