ARG51530

anti-JunB phospho (Ser79) antibody

anti-JunB phospho (Ser79) antibody for IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat

Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes JunB phospho (Ser79)
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name JunB
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of serine 79 (G-A-S(p)-L-K) derived from Human JunB.
Conjugation Un-conjugated
Alternate Names AP-1; Transcription factor jun-B

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:100
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16477 Mouse JUNB

GeneID: 24517 Rat JUNB

GeneID: 3726 Human JUNB

Gene Symbol JUNB
Gene Full Name jun B proto-oncogene
Background Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.
Function Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. [UniProt]
Research Area Gene Regulation antibody
Calculated MW 36 kDa
PTM Ubiquitinated by ITCH, leading to its degradation.

Images (2) Click the Picture to Zoom In

  • ARG51530 anti-JunB phospho (Ser79) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51530 anti-JunB phospho (Ser79) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51530 anti-JunB phospho (Ser79) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with ARG51530 anti-JunB phospho (Ser79) antibody.