ARG51532

anti-JunD phospho (Ser255) antibody

anti-JunD phospho (Ser255) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes JunD phospho (Ser255)
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name JunD
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of serine 255 (G-E-S(p)-P-P) derived from Human JunD.
Conjugation Un-conjugated
Alternate Names AP-1; Transcription factor jun-D

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16478 Mouse JUND

GeneID: 24518 Rat JUND

GeneID: 3727 Human JUND

Gene Symbol JUND
Gene Full Name jun D proto-oncogene
Background Binds an AP-1 site and upon cotransfection stimulates the activity of a promoter that bears an AP-1 site.
Function Transcription factor binding AP-1 sites. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody
Calculated MW 35 kDa

Images (2) Click the Picture to Zoom In

  • ARG51532 anti-JunD phospho (Ser255) antibody WB image

    Western blot: Extracts from 293 cells stained with ARG51532 anti-JunD phospho (Ser255) antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).

  • ARG51532 anti-JunD phospho (Ser255) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51532 anti-JunD phospho (Ser255) antibody (left) or the same antibody preincubated with blocking peptide (right).