ARG53984

anti-KDM1 / LSD1 antibody

anti-KDM1 / LSD1 antibody for Western blot,ICC/IF,Immunoprecipitation and Human,Monkey

Gene Regulation antibody

Overview

Product Description Mouse Monoclonal antibody recognizes KDM1A
Tested Reactivity Hu, Mk
Tested Application ICC/IF, IP, WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name KDM1 / LSD1
Antigen Species Human
Immunogen Purified recombinant human KDM1/LSD1 protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names Flavin-containing amine oxidase domain-containing protein 2; Lysine-specific histone demethylase 1A; EC 1.-.-.-; BRAF35-HDAC complex protein BHC110; AOF2; KDM1; LSD1; BHC110

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 110 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 3 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23028 Human KDM1A

Swiss-port # O60341 Human Lysine-specific histone demethylase 1A

Gene Symbol KDM1A
Gene Full Name lysine (K)-specific demethylase 1A
Background Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3,thereby acting as a coactivator or a corepressor,depending on the context.Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.Acts as a corepressor by mediating demethylation of H3K4me,a specific tag for epigenetic transcriptional activation.Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me.May play a role in the repression of neuronal genes.Alone,it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity.Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription,by being recruited to ANDR target genes and mediating demethylation of H3K9me,a specific tag for epigenetic transcriptional repression.The presence of PRKCB in ANDR-containing complexes,which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph),a specific tag that prevents demethylation H3K4me,prevents H3K4me demethylase activity of KDM1A.Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation.Demethylates and stabilizes the DNA methylase DNMT1.Required for gastrulation during embryogenesis.Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses,via histone deacetylase (HDAC) recruitment,a number of genes implicated in multilineage blood cell development.Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1,CDN7 and KRT8.Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.
Function Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7. [UniProt]
Cellular Localization Nucleus
Research Area Gene Regulation antibody
Calculated MW 93 kDa
PTM Polyubiquitinated by JADE2; which leads to its proteasomal degradation.

Images (3) Click the Picture to Zoom In

  • ARG53984 anti-KDM1 / LSD1 antibody ICC/IF image

    Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with ARG53984 anti-KDM1 / LSD1 antibody at 1:100 dilution.

  • ARG53984 anti-KDM1 / LSD1 antibody WB image

    Western blot: HCT116 cell lysate stained with ARG53984 anti-KDM1 / LSD1 antibody at 1:1000 dilution.

  • ARG53984 anti-KDM1 / LSD1 antibody IP image

    Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG53984 anti-KDM1 / LSD1 antibody.