ARG40757

anti-KDM3A antibody

anti-KDM3A antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes KDM3A
Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name KDM3A
Antigen Species Human
Immunogen Synthetic peptide within aa. 350-450 of Human KDM3A (NP_060903.2).
Conjugation Un-conjugated
Full Name lysine (K)-specific demethylase 3A
Alternate Names JMJD1; JHDM2A; Jumonji domain-containing protein 1A; JmjC domain-containing histone demethylation protein 2A; JMJD1A; Lysine-specific demethylase 3A; EC 1.14.11.-; TSGA; JHMD2A

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control 293T
Calculated MW 147 kDa
Observed Size 147 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 104263 Mouse KDM3A

GeneID: 55818 Human KDM3A

Swiss-port # Q6PCM1 Mouse Lysine-specific demethylase 3A

Swiss-port # Q9Y4C1 Human Lysine-specific demethylase 3A

Gene Symbol KDM3A
Gene Full Name lysine (K)-specific demethylase 3A
Background This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
Function Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. [UniProt]
Cellular Localization Cytoplasm. Nucleus. Note=Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa (By similarity). [UniProt]
Highlight Related products:
KDM3A antibodies; Anti-Rabbit IgG secondary antibodies;
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Images (2) Click the Picture to Zoom In

  • ARG40757 anti-KDM3A antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse brain stained with ARG40757 anti-KDM3A antibody at 1:100 dilution.

  • ARG40757 anti-KDM3A antibody WB image

    Western blot: 25 µg of 293T cell lysate stained with ARG40757 anti-KDM3A antibody at 1:1000 dilution.