ARG54857

anti-KDM6B / JMJD3 antibody

anti-KDM6B / JMJD3 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human

Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes KDM6B / JMJD3
Tested Reactivity Hu
Predict Reactivity Ms
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name KDM6B / JMJD3
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 1-30 (N-terminus) of Human JMJD3.
Conjugation Un-conjugated
Alternate Names JmjC domain-containing protein 3; Lysine-specific demethylase 6B; JMJD3; Lysine demethylase 6B; Jumonji domain-containing protein 3; EC 1.14.11.-

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:10 - 1:50
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23135 Human KDM6B

Swiss-port # O15054 Human Lysine-specific demethylase 6B

Gene Symbol KDM6B
Gene Full Name lysine (K)-specific demethylase 6B
Function Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. [UniProt]
Cellular Localization Nucleus.
Highlight Related products:
KDM6B antibodies; Anti-Rabbit IgG secondary antibodies;
Related news:
Hypoxia-induced transcription, histone demethylases are involved
Research Area Gene Regulation antibody
Calculated MW 177 kDa

Images (3) Click the Picture to Zoom In

  • ARG54857 anti-KDM6B / JMJD3 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG54857 anti-KDM6B / JMJD3 antibody (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI (blue) for nuclear staining.

  • ARG54857 anti-KDM6B / JMJD3 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human brain tissue stained with ARG54857 anti-KDM6B / JMJD3 antibody.

  • ARG54857 anti-KDM6B / JMJD3 antibody WB image

    Western blot: HeLa cell lysate stained with ARG54857 anti-KDM6B / JMJD3 antibody.