ARG63601
anti-KLF1 / EKLF antibody
anti-KLF1 / EKLF antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes KLF1 / EKLF |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Cow, Dog, Pig |
| Tested Application | IHC-P |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | KLF1 / EKLF |
| Antigen Species | Human |
| Immunogen | ATAETALPSISTLT-C |
| Conjugation | Un-conjugated |
| Alternate Names | Krueppel-like factor 1; EKLF; CDAN4; INLU; Erythroid krueppel-like transcription factor; HBFQTL6 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 38 kDa |
| PTM | Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity (By similarity). Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex (By similarity). Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity (By similarity). |
Images (1) Click the Picture to Zoom In
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ARG63601 anti-KLF1 / EKLF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human bone marrow tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG63601 anti-KLF1 / EKLF antibody at 4 µg/ml dilution followed by HRP-staining.
