ARG46145
anti-KLHL25 antibody
anti-KLHL25 antibody for Western blot,ICC/IF and Human
Overview
| Product Description | Chicken Polyclonal antibody recognizes KLHL25 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Chicken |
| Clonality | Polyclonal |
| Isotype | IgY |
| Target Name | KLHL25 |
| Antigen Species | Human |
| Immunogen | A 14 amino acid synthetic peptide within aa. 230 - 280 of human KLHL25. |
| Conjugation | Un-conjugated |
| Alternate Names | KLHL25; ENC-2; Kelch-like protein 25; ENC2; Ectoderm-neural cortex protein 2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Purification | Affinity chromatography purified |
|---|---|
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KLHL25 |
| Gene Full Name | kelch-like family member 25 |
| Function | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for translational homeostasis. The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E. [UniProt] |
| Calculated MW | 66 kDa |
Images (2) Click the Picture to Zoom In
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ARG46145 anti-KLHL25 antibody ICC/IF image
Immunofluorescence: Jurkat stained with ARG46145 anti-KLHL25 antibody.
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ARG46145 anti-KLHL25 antibody WB image
Western blot: Jurkat stained with ARG46145 anti-KLHL25 antibody.
