ARG21033

anti-KLRG1 antibody [2F1] (FITC)

anti-KLRG1 antibody [2F1] (FITC) for Flow cytometry,Western blot and Mouse,Human

Overview

Product Description FITC-conjugated Syrian Hamster Monoclonal antibody [2F1] recognizes KLRG1
Tested Reactivity Hu, Ms
Tested Application FACS, WB
Specificity The clone 2F1 reacts with mouse killer cell lectin-like receptor G1. The 2F1 antibody stains 30-60% of NK1.1+CD3- splenocytes, and a small fraction of T cells in all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL).
Host Syrian Hamster
Clonality Monoclonal
Clone 2F1
Isotype IgG2
Target Name KLRG1
Antigen Species Mouse
Immunogen IL-2-activated NK cells (A-LAK cells) from B6 (H-2b) mice
Conjugation FITC
Full Name killer cell lectin-like receptor subfamily G, member 1
Alternate Names 2F1; MAFA-L; C-type lectin domain family 15 member A; MAFA-2F1; MAFA-like receptor; CLEC15A; Killer cell lectin-like receptor subfamily G member 1; MAFA; ITIM-containing receptor MAFA-L; Mast cell function-associated antigen; MAFA-LIKE

Application Instructions

Application Suggestion
Tested Application Dilution
FACS< 1 µg/10^6 cells
WBAssay-dependent
Application Note WB: Under non-reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 22 kDa

Properties

Form Liquid
Buffer PBS and Sodium azide
Preservative Sodium azide
Concentration 0.5 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 10219 Human KLRG1

GeneID: 50928 Mouse KLRG1

Swiss-port # O88713 Mouse Killer cell lectin-like receptor subfamily G member 1

Swiss-port # Q96E93 Human Killer cell lectin-like receptor subfamily G member 1

Gene Symbol KLRG1
Gene Full Name killer cell lectin-like receptor subfamily G, member 1
Background Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. The protein encoded by this gene belongs to the killer cell lectin-like receptor (KLR) family, which is a group of transmembrane proteins preferentially expressed in NK cells. Studies in mice suggested that the expression of this gene may be regulated by MHC class I molecules. Alternatively spliced transcript variants have been reported, but their full-length natures have not yet been determined. [provided by RefSeq, Jul 2008]
Function Plays an inhibitory role on natural killer (NK) cells and T-cell functions upon binding to their non-MHC ligands. May mediate missing self recognition by binding to a highly conserved site on classical cadherins, enabling it to monitor expression of E-cadherin/CDH1, N-cadherin/CDH2 and R-cadherin/CDH4 on target cells. [UniProt]