anti-Ki-67 antibody

anti-Ki-67 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse,Rat

Microvascular Density Study antibody; Proliferation Marker antibody
publication_link Publication3


Product Description Rabbit Polyclonal antibody recognizes Ki-67
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Ki-67
Antigen Species Human
Immunogen Recombinant protein corresponding to aa. 1111-1490 of Human Ki-67.
Conjugation Un-conjugated
Alternate Names Antigen KI-67; MIB-; KIA; MIB-1; PPP1R105

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:2000 - 1:5000
WB1:5000 - 1:10000
Application Note IHC-P: IHC-P was tested in the publication reference (PMID: 34650054) and sodium citrate was used as antigen retrieval buffer
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Buffer Serum and 5 mM Sodium azide.
Preservative 5 mM Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 4288 Human MKI67

Swiss-port # P46013 Human Antigen KI-67

Gene Symbol MKI67
Gene Full Name marker of proliferation Ki-67
Background Ki-67 is a nuclear protein. It is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
Function Ki-67 required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). [UniProt]
Cellular Localization Chromosome. Nucleus, nucleolus. Note=Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface. Associates with satellite DNA in G1 phase. Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes. [UniProt]
Research Area Microvascular Density Study antibody; Proliferation Marker antibody
Calculated MW 359 kDa
PTM Phosphorylated. Hyperphosphorylated in mitosis. Hyperphosphorylated form does not bind DNA. [UniProt]

Images (5) Click the Picture to Zoom In

  • ARG11083 anti-Ki-67 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG11083 anti-Ki-67 antibody (red) at 1:5000 dilution and a mouse monoclonal antibody to fibrillarin at 1:2000 dilution (green). DAPI (blue) for nuclear staining.

    The Ki67 protein accumulates in and around the nucleoli of interphase cells such as those on the right, and the nucleoli are revealed by the fibrillarin antibody. In contrast, cells in the quiescent G0 state such as those on the left are Ki67 negative but fibrillarin positive.

  • ARG11083 anti-Ki-67 antibody ICC/IF image

    Immunofluorescence: NIH/3T3 cells stained with ARG11083 anti-Ki-67 antibody (red) at 1:5000 dilution and Mouse mAb to beta Tubulin (green) at 1:2000 dilution. Hoechst (blue) for nuclear staining.

    The Ki-67 antibody strongly stains the nuclei of dividing cells, but not quiescent cells.

  • ARG11083 anti-Ki-67 antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Human breast tissue including both normal and cancer cells stained with ARG11083 anti-Ki-67 antibody.

    The cancer cells divide rapidly and heavily express Ki-67 and so stain strongly with the Ki-67 antibody.

  • ARG11083 anti-Ki-67 antibody WB image

    Western blot: HeLa and HEK293 cell lysates stained with ARG11083 anti-Ki-67 antibody at 1:10000 dilution.

    Strong double bands larger than the 250 kDa standard correspond to full length 345 kDa and 395 kDa Ki67 isoforms, while smaller proteolytic fragments of these isoforms are also invariably detected on the blot.

  • ARG11083 anti-Ki-67 antibody IHC-Fr image

    Immunohistochemistry: Frozen section of adult mouse hippocampus dentate region stained with ARG11083 anti-Ki-67 antibody (red) at 1:2000 dilution and mouse monoclonal antibody to FOX3 / NeuN (green) at 1:2000 dilution. Hoechst dye (blue) staining of DNA. Bottom right image: All three merged.

    Dividing cells are very rare in adult animals, but one can be seen in the center of the image. Chromosomes can be seen in blue and their Ki-67 coating can be seen in red. The dividing cell is FOX3 / NeuN negative and so is presumably a glial cell.

Customer's Feedback

nuts_pic      Excellent

Review for anti-Ki-67 antibody


Sample:Mouse Skin

Sample Preparation Method:Paraffin

Fixation Buffer:4% PFA

Fixation Time:Overnight

Fixation Temperature:Room Temperature ºC

Antigen Retrieval Buffer:Citrate + Tween 20

Antigen Retrieval Time:30 min

Antigen Retrieval Temperature:95 ºC ºC

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

The Prostaglandin E2 Receptor EP4 Promotes Vascular Neointimal Hyperplasia through Translational Control of Tenascin C via the cAPM/PKA/mTORC1/rpS6 Pathway

ICC/IF / Rat

Hu Xu et al.
Cells,  (2022)




Long-term remission under Disitamab Vedotin (RC48) in HR-positive/HER2-positive metastatic breast cancer with brain meningeal, and bone marrow involvement: A case report

IHC-P / Human

Qifeng Wu et al.
Oncol Lett.,  (2022)




Diet-induced obesity accelerates oral carcinogenesis by recruitment and functional enhancement of myeloid-derived suppressor cells

IHC-P / Mouse

Jianmin Peng et al.
Cell Death Dis.,  (2021)