ARG46830
anti-Ki-67 antibody
anti-Ki-67 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse
Overview
| Product Description | Rat Monoclonal antibody recognizes Ki-67 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P |
| Host | Rat |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Target Name | Ki-67 |
| Antigen Species | Human |
| Immunogen | A synthesized peptide derived from human Ki-67 |
| Conjugation | Un-conjugated |
| Alternate Names | Antigen KI-67; MIB-; KIA; MIB-1; PPP1R105 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: High-pressure and temperature Sodium Citrate pH 6.0 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity chromatography purified |
| Buffer | PBS, 150mM NaCl and 0.02% Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MKI67 |
| Gene Full Name | marker of proliferation Ki-67 |
| Background | |anti Ki67 antibody SP6|MKI67|Antigen KI67|MIB1|marker of proliferation Ki67|KIA|MIB|PPP1R105|MKI67 SP6|SP6|MIB| |
| Function | Ki-67 required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). [UniProt] |
| Cellular Localization | Nucleus |
| Highlight | Related Antibody Duos and Panels: ARG30327 Microvascular Density Antibody Panel Related products: Ki-67 antibodies; Ki-67 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) |
| Calculated MW | 345 kDa and 395 kDa |
| PTM | Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA. |
Images (1) Click the Picture to Zoom In
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ARG46830 anti-Ki-67 antibody IHC-P image
Immunohistochemistry: Human liver cancer in Chimera mouse stained with ARG46830 anti-Ki-67 antibody.
