ARG10575

anti-Ki-67 antibody [E18-E]

anti-Ki-67 antibody [E18-E] for IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human

Overview

Product Description Rabbit Monoclonal antibody [E18-E] recognizes Ki-67
Tested Reactivity Hu
Tested Application ICC/IF, IHC-P
Host Rabbit
Clonality Monoclonal
Clone E18-E
Isotype IgG
Target Name Ki-67
Antigen Species Human
Immunogen Synthetic peptide around the N-terminus of Human Ki-67
Conjugation Un-conjugated
Full Name marker of proliferation Ki-67
Alternate Names Antigen KI-67; MIB-; KIA; MIB-1; PPP1R105

Application Instructions

Application Suggestion
Tested Application
ICC/IFAssay-dependent
IHC-P1:100 - 1:200
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 20 mM Tris-HCl (pH 8.0), 0.05% Sodium azide and 20 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 20 mg/ml BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 4288 Human MKI67

Swiss-port # P46013 Human Antigen KI-67

Gene Symbol MKI67
Gene Full Name marker of proliferation Ki-67
Background This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
Function Thought to be required for maintaining cell proliferation. [UniProt]
PTM Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.

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  • ARG10575 anti-Ki-67 antibody [E18-E] ICC/IF image

    Immunocytochemistry: HEK293 cells stained with ARG10575 anti-Ki-67 antibody [E18-E] at 1:100 dilution.

  • ARG10575 anti-Ki-67 antibody [E18-E] IHC-P image

    Immunohistochemistry: Formalin fixed, paraffin embedded Human plasma cell myeloma in the bone marrow biopsy (4 µm sections) stained with ARG10575 anti-Ki-67 antibody [E18-E].

  • ARG10575 anti-Ki-67 antibody [E18-E] IHC-P image

    Immunohistochemistry: Formalin fixed, paraffin embedded Human B-cell lymphoma showing high proliferating index (4 µm sections) stained with ARG10575 anti-Ki-67 antibody [E18-E].