ARG10575

anti-Ki-67 antibody [E18-E]

anti-Ki-67 antibody [E18-E] for IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human

Overview

Product Description Rabbit Monoclonal antibody [E18-E] recognizes Ki-67
Tested Reactivity Hu
Tested Application ICC/IF, IHC-P
Host Rabbit
Clonality Monoclonal
Clone E18-E
Isotype IgG
Target Name Ki-67
Antigen Species Human
Immunogen Synthetic peptide around the N-terminus of Human Ki-67
Conjugation Un-conjugated
Full Name marker of proliferation Ki-67
Alternate Names Antigen KI-67; MIB-; KIA; MIB-1; PPP1R105

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IHC-P1:100 - 1:200
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 359 kDa

Properties

Form Liquid
Buffer 20 mM Tris-HCl (pH 8.0), 0.05% Sodium azide and 20 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 20 mg/ml BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 4288 Human MKI67

Swiss-port # P46013 Human Antigen KI-67

Gene Symbol MKI67
Gene Full Name marker of proliferation Ki-67
Background Ki-67 is a nuclear protein. It is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
Function Ki-67 required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). [UniProt]
PTM Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.

Images (3) Click the Picture to Zoom In

  • ARG10575 anti-Ki-67 antibody [E18-E] ICC/IF image

    Immunocytochemistry: HEK293 cells stained with ARG10575 anti-Ki-67 antibody [E18-E] at 1:100 dilution.

  • ARG10575 anti-Ki-67 antibody [E18-E] IHC-P image

    Immunohistochemistry: Formalin fixed, paraffin embedded Human plasma cell myeloma in the bone marrow biopsy (4 µm sections) stained with ARG10575 anti-Ki-67 antibody [E18-E].

  • ARG10575 anti-Ki-67 antibody [E18-E] IHC-P image

    Immunohistochemistry: Formalin fixed, paraffin embedded Human B-cell lymphoma showing high proliferating index (4 µm sections) stained with ARG10575 anti-Ki-67 antibody [E18-E].