ARG54158

anti-Ku 70 antibody

anti-Ku 70 antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Monkey

Cancer antibody; Gene Regulation antibody
publication_link Publication1

Overview

Product Description Mouse Monoclonal antibody recognizes Ku 70
Tested Reactivity Hu, Mk
Tested Application ICC/IF, IP, WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name Ku 70
Antigen Species Human
Immunogen Purified recombinant human Ku70 protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names DNA repair protein XRCC6; Thyroid-lupus autoantigen; Lupus Ku autoantigen protein p70; EC 4.2.99.-; EC 3.6.4.-; ATP-dependent DNA helicase II 70 kDa subunit; X-ray repair complementing defective repair in Chinese hamster cells 6; CTC box-binding factor 75 kDa subunit; 70 kDa subunit of Ku antigen; CTC75; 5'-deoxyribose-5-phosphate lyase Ku70; KU70; TLAA; 5'-dRP lyase Ku70; CTCBF; ML8; G22P1; X-ray repair cross-complementing protein 6; ATP-dependent DNA helicase 2 subunit 1; Ku70

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 67 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2547 Human XRCC6

Swiss-port # P12956 Human X-ray repair cross-complementing protein 6

Gene Symbol XRCC6
Gene Full Name X-ray repair complementing defective repair in Chinese hamster cells 6
Background Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
Function Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. [UniProt]
Cellular Localization Nucleus. Chromosome
Research Area Cancer antibody; Gene Regulation antibody
Calculated MW 70 kDa
PTM Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.

Images (3) Click the Picture to Zoom In

  • ARG54158 anti-Ku 70 antibody ICC/IF image

    Immunofluorescence: HeLa cells fixed with -20°C Methanol and stained with ARG54158 anti-Ku 70 antibody at 1:200 dilution.

  • ARG54158 anti-Ku 70 antibody WB image

    Western blot: HeLa cell lysate stained with ARG54158 anti-Ku 70 antibody at 1:1000 dilution.

  • ARG54158 anti-Ku 70 antibody IP image

    Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54158 anti-Ku 70 antibody.

Specific References

Propolis alleviates 4-aminobiphenyl-induced oxidative DNA damage by inhibition of CYP2E1 expression in human liver cells

WB / Human

Eva Ari Wahyuni et al.
Environ Toxicol.,  (2021)

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