anti-Ku 70 antibody

anti-Ku 70 antibody for Western blot,ICC/IF,Immunoprecipitation and Human,Mouse,Monkey

publication_link Publication2


Product Description Rabbit Polyclonal antibody recognizes Ku 70
Tested Reactivity Hu, Ms, Mk
Tested Application ICC/IF, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Ku 70
Antigen Species Human
Immunogen Synthetic peptide of Human Ku70.
Conjugation Un-conjugated
Alternate Names DNA repair protein XRCC6; Thyroid-lupus autoantigen; Lupus Ku autoantigen protein p70; EC 4.2.99.-; EC 3.6.4.-; ATP-dependent DNA helicase II 70 kDa subunit; X-ray repair complementing defective repair in Chinese hamster cells 6; CTC box-binding factor 75 kDa subunit; 70 kDa subunit of Ku antigen; CTC75; 5'-deoxyribose-5-phosphate lyase Ku70; KU70; TLAA; 5'-dRP lyase Ku70; CTCBF; ML8; G22P1; X-ray repair cross-complementing protein 6; ATP-dependent DNA helicase 2 subunit 1; Ku70

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:100
IP1:20 - 1:50
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control SW480
Observed Size ~ 70 kDa


Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 14375 Mouse XRCC6

GeneID: 2547 Human XRCC6

Swiss-port # P12956 Human X-ray repair cross-complementing protein 6

Swiss-port # P23475 Mouse X-ray repair cross-complementing protein 6

Gene Symbol XRCC6
Gene Full Name X-ray repair complementing defective repair in Chinese hamster cells 6
Background The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]
Function Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. [UniProt]
Cellular Localization Chromosome, Nucleus. [UniProt]
Calculated MW 70 kDa
PTM Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG57851 anti-Ku 70 antibody ICC/IF image

    Immunofluorescence: A549 cells stained with ARG57851 anti-Ku 70 antibody.

  • ARG57851 anti-Ku 70 antibody WB image

    Western blot: 25 µg of SW480 cell lysate stained with ARG57851 anti-Ku 70 antibody at 1:1000 dilution.

  • ARG57851 anti-Ku 70 antibody IP image

    Immunoprecipitation: 100 µg extracts of SW480 cells were immunoprecipitated and stained with ARG57851 anti-Ku 70 antibody at 1:1000 dilition.

Specific References

miR-3059-3p Regulates Glioblastoma Multiforme Radiosensitivity Enhancement through the Homologous Recombination Pathway of DNA Repair

WB / Human

Yu-Wen Cheng et al.
J Oncol,  (2022)




Phospho-Ku70 induced by DNA damage interacts with RNA Pol II and promotes the formation of phospho-53BP1 foci to ensure optimal cNHEJ

WB / Human

Amelie Schellenbauer et al.
Nucleic Acids Res.,  (2021)