ARG54669

anti-LC3C antibody

anti-LC3C antibody for ELISA,Immunohistochemistry,Western blot,ICC/IF and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes LC3C
Tested Reactivity Hu
Tested Application ELISA, ICC/IF, IHC, WB
Specificity MAP1LC3C antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3C are known to exist. MAP1LC3C antibody is predicted to not cross-react with MAP1LC3A or MAP1LC3B.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name LC3C
Immunogen Synthetic peptide (18 aa) within the last 50 aa of Human MAP1LC3C.
Conjugation Un-conjugated
Alternate Names Microtubule-associated proteins 1A/1B light chain 3C; MAP1A/MAP1B light chain 3 C; ATG8J; Microtubule-associated protein 1 light chain 3 gamma; MAP1 light chain 3-like protein 3; MAP1A/MAP1B LC3 C; Autophagy-related protein LC3 C; LC3C; Autophagy-related ubiquitin-like modifier LC3 C

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-Dependent
ICC/IF20 μg/mL
IHC5 μg/mL
WB1 - 2 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human Brain Tissue Lysate
Observed Size 16 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 440738 Human MAP1LC3C

Swiss-port # Q9BXW4 Human Microtubule-associated proteins 1A/1B light chain 3C

Gene Symbol MAP1LC3C
Gene Full Name microtubule-associated protein 1 light chain 3 gamma
Background Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3C belongs to the MAP1 LC3 family of proteins that form mature complexes with MAP1A and MAP1B which are thought to be important in the formation and development of axons and dendrites (2). MAP1LC3C is one of three isoforms of MAP1LC3, the mammalian homolog of yeast ATG8, an essential autophagy protein. These isoforms exhibit distinct expression patterns and MAP1LC3C, like MAP1LC3A but not MAP1LC3B, is post-translationally modified, suggesting the three isoforms may have different physiological functions (3). |
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 17 kDa
PTM The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II (PubMed:15187094).
The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.

Images (3) Click the Picture to Zoom In

  • ARG54669 anti-MAP1LC3C antibody ICC/IF image

    Immunofluorescence: human brain tissue stained with ARG54669 anti-MAP1LC3C antibody at 20 μg/ml.

  • ARG54669 anti-MAP1LC3C antibody IHC image

    Immunohistochemistry: human brain tissue stained with ARG54669 anti-MAP1LC3C antibody at 5 μg/ml.

  • ARG54669 anti-MAP1LC3C antibody WB image

    Western blot: human brain tissue lysate stained with ARG54669 anti-MAP1LC3C antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.