ARG64029

anti-LEF1 antibody

anti-LEF1 antibody for Western blot and Human,Rat

Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes LEF1
Tested Reactivity Hu, Rat
Predict Reactivity Ms, Dog, Zfsh
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name LEF1
Antigen Species Human
Immunogen C-QHEQRKEQEPKRPH
Conjugation Un-conjugated
Alternate Names Lymphoid enhancer-binding factor 1; TCF1-alpha; TCF7L3; T cell-specific transcription factor 1-alpha; LEF-1; TCF1ALPHA; TCF10

Application Instructions

Application Note WB: Recommend incubate at RT for 1h. Approx 48 kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2 kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al , EMBO J. 2002 Jun 3;21(11):2703-14.; PMID: 12032083). The same band was also observed in Mouse and Rat Spleen lysates. Recommended concentration: 1-3 µg/ml.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 161452 Rat LEF1

GeneID: 51176 Human LEF1

Swiss-port # Q9QXN1 Rat Lymphoid enhancer-binding factor 1

Swiss-port # Q9UJU2 Human Lymphoid enhancer-binding factor 1

Background This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
Research Area Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
PTM Phosphorylated at Thr-155 and/or Ser-166 by NLK. Phosphorylation by NLK at these sites represses LEF1-mediated transcriptional activation of target genes of the canonical Wnt signaling pathway.

Images (1) Click the Picture to Zoom In

  • ARG64029 anti-LEF1 antibody WB image

    Western Blot: Daudi cell lysate (35 µg protein in RIPA buffer) stained with ARG64029 anti-LEF1 antibody at 1 µg/ml dilution.