anti-LEF1 antibody [SQab19160]

anti-LEF1 antibody [SQab19160] for IHC-Formalin-fixed paraffin-embedded sections and Human


Product Description Recombinant Rabbit Monoclonal antibody [SQab19160] recognizes LEF1
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SQab19160
Isotype IgG
Target Name LEF1
Antigen Species Human
Immunogen Synthetic peptide within aa. 100-200 of Human LEF1.
Conjugation Un-conjugated
Alternate Names Lymphoid enhancer-binding factor 1; TCF1-alpha; TCF7L3; T cell-specific transcription factor 1-alpha; LEF-1; TCF1ALPHA; TCF10

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 51176 Human LEF1

Swiss-port # Q9UJU2 Human Lymphoid enhancer-binding factor 1

Gene Symbol LEF1
Gene Full Name lymphoid enhancer-binding factor 1
Background This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
Function Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. [UniProt]
Cellular Localization Nucleus. Note=Found in nuclear bodies upon PIASG binding. [UniProt]
Calculated MW 44 kDa
PTM Phosphorylated at Thr-155 and/or Ser-166 by NLK. Phosphorylation by NLK at these sites represses LEF1-mediated transcriptional activation of target genes of the canonical Wnt signaling pathway. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG66675 anti-LEF1 antibody [SQab19160] IHC-P image

    Immunohistochemistry: Paraffin-embedded Human Hodgkin's lymphoma tissue stained with ARG66675 anti-LEF1 antibody [SQab19160].

  • ARG66675 anti-LEF1 antibody [SQab19160] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human tonsil tissue stained with ARG66675 anti-LEF1 antibody [SQab19160]. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).