ARG47135
anti-LIMCH1 antibody
anti-LIMCH1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody recognizes LIMCH1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | LIMCH1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human LIMCH1. |
| Conjugation | Un-conjugated |
| Alternate Names | LIMCH1; LIM and calponin homology domains-containing protein 1; KIAA1102 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A |
| Buffer | PBS, 0.09% Sodium azide, 50% Glycerol and 0.5% BSA |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9UPQ0 Human LIM and calponin homology domains-containing protein 1 |
|---|---|
| Gene Symbol | LIMCH1 |
| Gene Full Name | LIM and calponin homology domains 1 |
| Background | Enables myosin II head/neck binding activity. Involved in several processes, including cytoplasmic actin-based contraction involved in cell motility; positive regulation of stress fiber assembly; and regulation of focal adhesion assembly. Located in myosin II complex and stress fiber. [provided by Alliance of Genome Resources, Jul 2025] |
| Function | Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration. [UniProt] |
| Cellular Localization | Cytoplasm; Cytoskeleton. [UniProt] |
| Calculated MW | 122 kDa |
| PTM | Phosphoprotein. [UniProt] |
