ARG53248

anti-LMO2 antibody [SP51]

anti-LMO2 antibody [SP51] for IHC-Formalin-fixed paraffin-embedded sections,Western blot,Flow cytometry and Human

Cell Biology and Cellular Response antibody

Overview

Product Description Rabbit Monoclonal antibody [SP51] recognizes LMO2
Tested Reactivity Hu
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SP51
Isotype IgG
Target Name LMO2
Antigen Species Human
Immunogen Synthetic peptide corresponding to near N-terminus of human LMO2 protein.
Conjugation Un-conjugated
Alternate Names RHOM2; LMO-2; TTG2; LIM domain only protein 2; T-cell translocation protein 2; RBTN2; Rhombotin-2; Cysteine-rich protein TTG-2; RBTNL1

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-Dependent
IHC-P1:100
WB1:25
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 30 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Hodgkin's Lymphoma, Tonsil, Ramos

Properties

Form Liquid
Purification Purified by protein A/G
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4005 Human LMO2

Swiss-port # P25791 Human Rhombotin-2

Background LMO2 encodes a cysteine-rich, two LIM-doman protein that is required for yolk sac erythropoiesis. The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where T-cell acute lymphoblastic leukemia-specific translocations occur.
Cellular Localization Nucleus
Research Area Cell Biology and Cellular Response antibody
Calculated MW 18 kDa

Images (2) Click the Picture to Zoom In

  • ARG53248 anti-LMO2 antibody [SP51] WB image

    Western Blot: Ramos stained with LMO2 antibody [SP51] (ARG53248)

  • ARG53248 anti-LMO2 antibody [SP51] IHC-P image

    Immunohistochemistry: Human Tonsil stained with LMO2 antibody [SP51] (ARG53248)