ARG54850

anti-LPAR2 / EDG4 antibody

anti-LPAR2 / EDG4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Gene Regulation antibody; Signaling Transduction antibody

Overview

Product Description

Rabbit Polyclonal antibody recognizes LPAR2 / EDG4

Tested Reactivity Hu
Predict Reactivity Mk
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name LPAR2 / EDG4
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 3-32 (N-terminus) of Human EDG4.
Conjugation Un-conjugated
Alternate Names LPA2; EDG4; Lysophosphatidic acid receptor Edg-4; EDG-4; Lysophosphatidic acid receptor 2; LPA receptor 2; LPA-2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control MDA-MB-231

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 9170 Human LPAR2

Swiss-port # Q9HBW0 Human Lysophosphatidic acid receptor 2

Gene Symbol LPAR2
Gene Full Name lysophosphatidic acid receptor 2
Background This gene encodes a member of family I of the G protein-coupled receptors, as well as the EDG family of proteins. This protein functions as a lysophosphatidic acid (LPA) receptor and contributes to Ca2+ mobilization, a critical cellular response to LPA in cells, through association with Gi and Gq proteins. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]
Function Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Plays a key role in phospholipase C-beta (PLC-beta) signaling pathway. Stimulates phospholipase C (PLC) activity in a manner that is independent of RALA activation. [UniProt]
Cellular Localization Cell surface. Cell membrane; Multi-pass membrane protein. Note=Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA colocalizes with RALA in the endocytic vesicles
Research Area Gene Regulation antibody; Signaling Transduction antibody
Calculated MW 39 kDa

Images (2) Click the Picture to Zoom In

  • ARG54850 anti-LPAR2 / EDG4 antibody (N-term) IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer tissue stained with ARG54850 anti-LPAR2 / EDG4 antibody (N-term).

  • ARG54850 anti-LPAR2 / EDG4 antibody (N-term) WB image

    Western blot: 35 µg of MDA-MB-231 cell lysate stained with ARG54850 anti-LPAR2 / EDG4 antibody (N-term) at 1:1000 dilution.