anti-Lamin A + C antibody
anti-Lamin A + C antibody for Western blot,ICC/IF,IHC-Frozen sections and Human,Horse,Cow,Pig,Rat,Mouse
|Product Description||Rabbit Polyclonal antibody recognizes Lamin A + C|
|Tested Reactivity||Hu, Ms, Rat, Cow, Hrs, Pig|
|Tested Application||ICC/IF, IHC-Fr, WB|
|Target Name||Lamin A + C|
|Immunogen||Full length recombinant Human Lamin A purified from E. coli.|
|Alternate Names||HGPS; Renal carcinoma antigen NY-REN-32; LDP1; FPL; LMN1; CDCD1; LMNL1; CDDC; PRO1; EMD2; CMT2B1; 70 kDa lamin; LFP; Prelamin-A/C; LMNC; FPLD2; LGMD1B; IDC; FPLD; CMD1A|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||lamin A/C|
|Background||The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]|
|Function||Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation. Required for osteoblastogenesis and bone formation. Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone.
Prelamin-A/C can accelerate smooth muscle cell senescence. It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence. [UniProt]
|Calculated MW||Lamin A: 74 kDa
Lamin C: 65 kDa
|PTM||Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Proteolytic cleavage of the C-terminal of 18 residues of prelamin-A/C results in the production of lamin-A/C. The prelamin-A/C maturation pathway includes farnesylation of CAAX motif, ZMPSTE24/FACE1 mediated cleavage of the last three amino acids, methylation of the C-terminal cysteine and endoproteolytic removal of the last 15 C-terminal amino acids. Proteolytic cleavage requires prior farnesylation and methylation, and absence of these blocks cleavage.
Sumoylation is necessary for the localization to the nuclear envelope.
Farnesylation of prelamin-A/C facilitates nuclear envelope targeting.
Images (2) Click the Picture to Zoom In
ARG10718 anti-Lamin A + C antibody ICC/IF image
Immunocytochemistry: HeLa cells stained with ARG10718 anti-Lamin A + C antibody (red) and co-stained with a monoclonal 6H11 to HSP27 (green) and DNA (blue). ARG10718 reveals strong nuclear lamina staining, while the Clone 6H11 reveals strong cytoplasmic staining. Since both DNA (blue) and Lamin A/C (red) are associated with the nuclear compartment, this region appears crimson in this image.
ARG10718 anti-Lamin A + C antibody WB image
Western blot: Strip crude HeLa cell extract stained with ARG10718 anti-Lamin A + C antibody. Note two strong and clean bands at 74 kDa and 65 kDa, corresponding to Lamin A and C.