ARG10736

anti-Laminin 111 antibody

anti-Laminin 111 antibody for ICC/IF,IHC-Frozen sections,Western blot and Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Laminin 111
Tested Reactivity Ms, Rat
Tested Application ICC/IF, IHC-Fr, WB
Specificity ARG10736 Laminin-111 antibody recognizes 3 laminin isotypes: α1 (440kDa), β1 (220kD) and γ1 (220kDa).
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Laminin 111
Antigen Species Mouse
Immunogen Laminin isolated from Mouse EHS cells.
Conjugation Un-conjugated
Full Name laminin, alpha 1; laminin, beta 1; laminin, gamma 1
Alternate Names LAMA; PTBHS; S-LAM-alpha; Laminin subunit alpha-1; laminin, beta 1; laminin, gamma 1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000 - 1:5000
IHC-Fr1:1000 - 1:5000
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 337 kDa

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 16772 Mouse LAMA1

Swiss-port # P19137 Mouse Laminin subunit alpha-1

Gene Symbol Lama1
Gene Full Name laminin, alpha 1; laminin, beta 1; laminin, gamma 1
Background This gene encodes one of the alpha 1 subunits of laminin. The laminins are a family of extracellular matrix glycoproteins that have a heterotrimeric structure consisting of an alpha, beta and gamma chain. These proteins make up a major component of the basement membrane and have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Mutations in this gene may be associated with Poretti-Boltshauser syndrome. [provided by RefSeq, Sep 2014]
Function Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. [UniProt]
Cellular Localization Secreted › extracellular space › extracellular matrix › basement membrane
Note: Major component.
PTM Tyrosine phosphorylated by PKDCC/VLK.

Images (3) Click the Picture to Zoom In

  • ARG10736 anti-Laminin 111 antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Rat brain stem stained with ARG10736 anti-Laminin 111 antibody (red) at 1:1000 dilution and costained with ARG52344 anti-Myelin Basic Protein antibody (green) at 1:5000 dilution. DAPI (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies.)

    The Laminin antibody is an excellent marker of basement membranes surrounding blood vessels, while the MBP antibody stains the myelin sheathes around axons.

  • ARG10736 anti-Laminin 111 antibody IHC-Fr image

    Immunohistochemistry: Frozen sections of Mouse cortex stained with ARG10736 anti-Laminin 111 antibody (red). Blue is DAPI staining of DNA. This antibody reveals strong staining in the basement membranes of blood vessels.

  • ARG10736 anti-Laminin 111 antibody WB image

    Western blot: 1) Rat heart cells lysates, and 2) 0.2 µg of purified laminin-111 protein from Mouse EHS sarcoma was stained with ARG10736 anti-Laminin 111 antibody. This antibody recognizes 3 laminin isotypes: α1 (440 kDa), β1 (220kD) and γ1 (220 kDa). Also recognized a laminin binding protein at 120 kDa in both Rat heart lysates and purified laminin protein. Since this protein always coexpresses with laminin this cross reactivity is irrelevant.