ARG63575
anti-MAML1 / Mastermind antibody
anti-MAML1 / Mastermind antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Developmental Biology antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes MAML1 / Mastermind |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MAML1 / Mastermind |
| Antigen Species | Human |
| Immunogen | VLPTCPMAEFALPR |
| Conjugation | Un-conjugated |
| Alternate Names | Mam1; Mastermind-like protein 1; Mam-1 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This protein is the human homolog of mastermind, a Drosophila protein that plays a role in the Notch signaling pathway involved in cell-fate determination. There is in vitro evidence that the human homolog forms a complex with the intracellular portion of human Notch receptors and can increase expression of a Notch-induced gene. This evidence supports its proposed function as a transcriptional co-activator in the Notch signaling pathway. [provided by RefSeq, Jul 2008] |
| Research Area | Developmental Biology antibody; Signaling Transduction antibody |
| Calculated MW | 108 kDa |
Images (2) Click the Picture to Zoom In
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ARG63575 anti-MAML1 / Mastermind antibody WB image
Western Blot: Human Skeletal Muscle lysate (35 µg protein in RIPA buffer) stained with ARG63575 anti-MAML1 / Mastermind antibody at 0.1 µg/ml dilution.
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ARG63575 anti-MAML1 / Mastermind antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Placenta. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63575 anti-MAML1 / Mastermind antibody at 3.8 µg/ml dilution followed by AP-staining.
