ARG45752

anti-MAP4K3 antibody

anti-MAP4K3 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes MAP4K3
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MAP4K3
Antigen Species Human
Immunogen Recombinant protein containing to human MAP4K3.
Conjugation Un-conjugated
Alternate Names MAP4K3; Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3; GLK; MAPKKKK3; RAB8IPL1; Germinal Center Kinase-Related Protein Kinase; MAPK/ERK Kinase Kinase Kinase 3; MEK Kinase Kinase 3; EC 2.7.11.1  47; MEKKK 3; Germinal Center Kinase-Like Kinase; EC 2.7.11 47; MEKKK3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 100 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 170920 Rat MAP4K3

GeneID: 225028 Mouse MAP4K3

GeneID: 8491 Human MAP4K3

Gene Symbol MAP4K3
Gene Full Name Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3
Background This gene encodes a member of the mitogen-activated protein kinase kinase kinase kinase family. The encoded protein activates key effectors in cell signalling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
Function Serine/threonine kinase that plays a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. [UniProt]
Cellular Localization Cytoplasm. [UniProt]
Calculated MW 101 kDa
PTM Acetylation; Phosphoprotein. [UniProt]

Images (5) Click the Picture to Zoom In

  • ARG45752 anti-MAP4K3 antibody IHC-P image

    Immunohistochemistry: Human skeletal muscle stained with ARG45752 anti-MAP4K3 antibody at 2 μg/ml dilution.

  • ARG45752 anti-MAP4K3 antibody WB image

    Western blot: HepG2 stained with ARG45752 anti-MAP4K3 antibody at 0.5 μg/ml dilution.

  • ARG45752 anti-MAP4K3 antibody FACS image

    Flow Cytometry: 293T stained with ARG45752 anti-MAP4K3 antibody at 1 µg/10^6 cells dilution.

  • ARG45752 anti-MAP4K3 antibody WB image

    Western blot: Rat brain stained with ARG45752 anti-MAP4K3 antibody at 0.5 μg/ml dilution.

  • ARG45752 anti-MAP4K3 antibody WB image

    Western blot: Mouse brain stained with ARG45752 anti-MAP4K3 antibody at 0.5 μg/ml dilution.