ARG64709
anti-MARCH10 antibody
anti-MARCH10 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Cell Biology and Cellular Response antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes MARCH10 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Predict Reactivity | Cow |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_001094345.1; NP_689811.2), which are identical. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MARCH10 |
| Antigen Species | Human |
| Immunogen | C-SRNYPQPRTEE |
| Conjugation | Un-conjugated |
| Alternate Names | Probable E3 ubiquitin-protein ligase MARCH10; EC 6.3.2.-; Membrane-associated RING finger protein 10; RNF190; MARCH-X; Membrane-associated RING-CH protein X; RING finger protein 190 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q5XIV2 Rat Probable E3 ubiquitin-protein ligase MARCH10 Swiss-port # Q8NA82 Human Probable E3 ubiquitin-protein ligase MARCH10 |
|---|---|
| Background | MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010] |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 91 kDa |
Images (2) Click the Picture to Zoom In
-
ARG64709 anti-MARCH10 antibody WB image
Western Blot: Rat Testis lysate (35 µg protein in RIPA buffer) stained with ARG64709 anti-MARCH10 antibody at 0.3 µg/ml dilution.
-
ARG64709 anti-MARCH10 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64709 anti-MARCH10 antibody at 5 µg/ml dilution followed by AP-staining.
