anti-MARCKS phospho (Ser152 / Ser156) antibody

anti-MARCKS phospho (Ser152 / Ser156) antibody for Western blot and Rat

Cell Biology and Cellular Response antibody; Signaling Transduction antibody


Product Description Rabbit Polyclonal antibody recognizes MARCKS phospho (Ser152 / Ser156)
Tested Reactivity Rat
Predict Reactivity Hu, Ms, Bov, Chk, Xenopus laevis, Zfsh
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MARCKS
Antigen Species Rat
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser152/156 conjugated to KLH
Conjugation Un-conjugated
Alternate Names MACS; 80K-L; Myristoylated alanine-rich C-kinase substrate; PKCSL; Protein kinase C substrate, 80 kDa protein, light chain; 80K-L protein; MARCKS; PRKCSL

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note Specific for the ~87k MARCKS protein phosphorylated at Ser152 and Ser156 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by λ-phosphatase
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 25603 Rat MARCKS

Gene Symbol Marcks
Gene Full Name myristoylated alanine rich protein kinase C substrate
Background Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al., 1990). The phosphorylation of Ser152/156 can be used as a measure of PKC activation although these sites are also phosphorylated by PRK1 (Palmer et al., 1996) MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994).
Highlight Related Antibody Duos and Panels:
ARG30216 Phospho MARCKS Antibody Duo for Rat (Total, pS152/156)
Related products:
MARCKS antibodies; MARCKS Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Calculated MW 32 kDa
PTM Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.

Images (1) Click the Picture to Zoom In

  • ARG52330 anti-MARCKS phospho (Ser152 / Ser156) antibody WB image

    Western blot: Rat brain lysate showing phospho-specific immunolabeling of the ~87k MARCKS protein stained with ARG52330 anti-MARCKS phospho (Ser152 / Ser156) antibody.