ARG51822

anti-MARCKS phospho (Ser170) antibody

anti-MARCKS phospho (Ser170) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human

Cell Biology and Cellular Response antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes MARCKS phospho (Ser170)
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MARCKS
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of Serine 170 (G-F-S(p)-F-K) derived from Human MARCKS.
Conjugation Un-conjugated
Alternate Names MACS; 80K-L; Myristoylated alanine-rich C-kinase substrate; PKCSL; Protein kinase C substrate, 80 kDa protein, light chain; 80K-L protein; MARCKS; PRKCSL

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4082 Human MARCKS

Swiss-port # P29966 Human Myristoylated alanine-rich C-kinase substrate

Gene Symbol MARCKS
Gene Full Name myristoylated alanine-rich protein kinase C substrate
Background MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Function MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Calculated MW 32 kDa
PTM Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.

Images (3) Click the Picture to Zoom In

  • ARG51822 anti-MARCKS phospho (Ser170) antibody WB image

    Western blot: Extracts from MCF cells untreated or treated with EGF stained with ARG51822 anti-MARCKS phospho (Ser170) antibody.

  • ARG51822 anti-MARCKS phospho (Ser170) antibody WB image

    Western blot: Extracts from 3T3 cells, treated with serum or calf intestinal phosphatase (CIP), stained with ARG51822 anti-MARCKS phospho (Ser170) antibody.

  • ARG51822 anti-MARCKS phospho (Ser170) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung carcinoma tissue stained with ARG51822 anti-MARCKS phospho (Ser170) antibody (left) or the same antibody preincubated with blocking peptide (right).